Biotechnology Kit© Teacher Resources

PCR - Sanger Sequencing - Restriction Enzymes

 

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PCR Activity

In this multi-part activity, students will explore the chemical mechanisms involved in PCR and how it can be used to identify diseases and other biotechnological markers.

Sanger Sequencing Activity

Students will identify the steps of the Sanger method of DNA sequencing, model the Sanger process - including the directionality (5’ and 3’ ends) of the primers and single-stranded PCR products, and more.

Restriction Enzymes

Students will explain the function of restriction enzymes and the role of restriction sites along the DNA, demonstrate the location of endonuclease cuts based upon a given sequence, model how a restriction enzyme will make cuts in a segment of DNA, and more.

Next Generation Science Standards

Connections to: A Framework for K-12 Science Education Practices, Crosscutting Concepts, and Core Ideas